Department of Chemical Engineering, Tokyo Institute of Technology, Tokyo, Japan.
J Basic Microbiol. 2014 May;54(5):350-7. doi: 10.1002/jobm.201200350. Epub 2013 May 17.
We evaluated whether the glucosamine content in the filamentous fungus Monascus ruber NBRC 32318, cultured on a solid surface (agar) containing different carbon and nitrogen sources, could be used as a measure of biomass. The relationship between the amounts of glucosamine and biomass was independent of the cultivation period, but was dependent on the carbon source (D-glucose, D-fructose, maltose, sucrose, or rice starch) and the nitrogen source (ammonium chloride, sodium nitrate, monosodium glutamate, or yeast extract) in the agar; it was also dependent on the culture method (solid-surface culture or submerged culture). We concluded that the amount of glucosamine extracted from M. ruber is a useful index for the fungal biomass when the relationship between M. ruber biomass and glucosamine content has previously been calibrated for the carbon and nitrogen sources used. Examination of microphotographs of M. ruber hyphae in conjunction with quantification of the glucosamine and biomass contents indicated that the variation in the glucosamine content per unit biomass affects the hyphal morphology of the fungus, and especially the hyphal diameter.
我们评估了在含有不同碳源和氮源的固体表面（琼脂）上培养的红曲菌 Monascus ruber NBRC 32318 中的葡糖胺含量是否可以用作生物量的衡量标准。葡糖胺和生物量之间的关系不依赖于培养时间，但依赖于琼脂中的碳源（D-葡萄糖、D-果糖、麦芽糖、蔗糖或大米淀粉）和氮源（氯化铵、硝酸钠、谷氨酸单钠或酵母提取物）；它还依赖于培养方法（固体表面培养或浸没培养）。我们得出结论，当已经为所用的碳源和氮源校准了 M. ruber 生物量和葡糖胺含量之间的关系时，从 M. ruber 中提取的葡糖胺的量是真菌生物量的有用指标。结合葡糖胺和生物量含量的红曲菌菌丝体的显微照片检查表明，单位生物量的葡糖胺含量的变化会影响真菌的菌丝形态，特别是菌丝直径。
