Chemical Institute, Federal University of Goiás, Brazil.
College of Agronomy, Federal University of Goiás, Brazil.
Acta Sci Pol Technol Aliment. 2020 Oct-Dec;19(4):445-456. doi: 10.17306/J.AFS.0870.
The addition of fatty acids and other molecules to culture media may intensify the production of biomolecules, such as monascus pigments, however, few studies of this have been developed. Thus, the objective of the present study was to investigate the effects of adding sodium octanoate to the culture medium, with a view to increasing the synthesis and production of the pigments produced by Monascus ruber CCT 3802 on solid and submerged cultivations.
Monacus ruber CCT 3802 was cultivated on solid and submerged media supplemented with different concentrations of sodium octanoate. The radial growth rate of the colonies was obtained from the declivity of the linear regression of the radius of the colonies as a function of cultivation time and the kinetics of submerged cultivations were performed. The filtrate obtained was submitted to scanning spectrophotometry at a range from 350 to 550 nm and the color parameters were determined by using the CIELAB color system. The data were submitted to a univariate analysis of variance (ANOVA) and the means obtained for each treatment submitted to Tukey's test using Statistica version 5.0 software at a 5% level of significance.
Sodium octanoate exerted a strong influence on growth and pigment production in solid and submerged cultivations. The values for L*, a* and b* were positive for pigments produced, with regards to colors close to red and yellow. In the media supplemented with 1.0 mM and 1.5 mM of sodium octanoate, the production of red pigments became expressive from 48 hours-cultivation, increasing considerably from the second to the fourth days. This shows that supplementation with sodium octanoate provides a greater production of pigments in a shorter time interval than the control culture, which required 144 hours of cultivation to present a higher value for AU510nm, which directly influenced pigment productivity.
The addition of sodium octanoate exerted a significant influence on both microbial growth and pigment production in both solid and submerged cultivations. The supplementation of the submerged cultures with sodium octanoate was responsible for an expressive production of pigments in just 48 hours, whereas 144 hours were necessary in the absence of sodium octanoate. These results are promising for increasing the productivity of pigment production, including possibilities for application on an industrial scale.
在培养基中添加脂肪酸和其他分子可以增强生物分子的生产，如红曲色素，但对此的研究很少。因此，本研究的目的是研究在固体和浸没培养中添加辛酸钠对培养基的影响，以期增加红曲霉 CCT 3802 产生的色素的合成和生产。
在固体和浸没培养基中添加不同浓度的辛酸钠培养红曲霉 CCT 3802。从菌落半径随培养时间的线性回归斜率获得菌落的径向生长率，并进行浸没培养动力学研究。将获得的滤液在 350 至 550nm 的范围内进行扫描分光光度法，并使用 CIELAB 颜色系统确定颜色参数。将数据进行单因素方差分析（ANOVA），并对每个处理组的平均值进行 Tukey 检验，使用 Statistica 版本 5.0 软件在 5%的显著水平下进行。
辛酸钠对固体和浸没培养中的生长和色素生产有很强的影响。对于接近红色和黄色的颜色，色素产生的 L*、a和 b值为正。在添加 1.0mM 和 1.5mM 辛酸钠的培养基中，红色色素的生产从 48 小时培养开始表现出表达性，从第二天到第四天显著增加。这表明，与对照培养相比，添加辛酸钠可以在更短的时间间隔内产生更多的色素，对照培养需要 144 小时的培养才能表现出更高的 AU510nm 值，这直接影响了色素生产力。
添加辛酸钠对固体和浸没培养中的微生物生长和色素生产都有显著影响。在浸没培养中添加辛酸钠仅需 48 小时即可实现色素的大量生产，而在没有辛酸钠的情况下则需要 144 小时。这些结果有望提高色素生产的生产力，包括在工业规模上应用的可能性。
