School of Materials Science and Engineering, and Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China.
Carbohydr Polym. 2013 Jul 1;96(1):342-8. doi: 10.1016/j.carbpol.2013.04.008. Epub 2013 Apr 15.
A novel in situ forming polysaccharides/polypeptide hydrogel composed of naturally derived materials for applications as adhesive sealant and hemostatic material was developed via Michael addition crosslinking, taking advantage of its mild condition. Thiol-modified chitosan (CSS) was fast in situ crosslinked by an efficient polypeptide crosslinker (EPLM) which was prepared by introducing maleimide groups onto ε-polylysine. Gelation can happen swiftly within 15-215s depending on the CSS concentration, the degree of substitution (DS) of maleimide groups, and the molar ratio of maleimide group to thiol group. Results indicated that storage modulus of the hydrogel increased dramatically with the increase of CSS concentration and DS of maleimide. The obtained adhesive hydrogel had an adhesion strength 4 times higher than that of the commercial fibrin glue. Notably, it is non-toxic to L929 cells and exhibits excellent prompt hemostatic property. Polysaccharides/polypeptide structure designed here facilitates to improve both the biocompatibility and the adhesive property.
一种新型的基于天然材料的原位形成的多糖/多肽水凝胶，可作为黏合剂密封剂和止血材料，通过迈克尔加成交联来制备，该方法具有条件温和的优点。巯基改性壳聚糖（CSS）与高效多肽交联剂（EPLM）快速原位交联，该交联剂是通过在 ε-聚赖氨酸上引入马来酰亚胺基团制备得到的。根据 CSS 浓度、马来酰亚胺基团的取代度（DS）和马来酰亚胺基团与巯基基团的摩尔比，凝胶可在 15-215s 内迅速形成。结果表明，水凝胶的储能模量随 CSS 浓度和马来酰亚胺 DS 的增加而显著增加。所得到的黏附性水凝胶的黏附强度比商业纤维蛋白胶高 4 倍。值得注意的是，它对 L929 细胞无毒，具有优异的即时止血性能。多糖/多肽结构的设计有助于提高生物相容性和黏附性。
