Department of Orthopedic Surgery, Saint Louis University, St. Louis, MO, USA.
Cells Tissues Organs. 2013;197(6):496-504. doi: 10.1159/000348794. Epub 2013 May 14.
CD271 has been applied to isolate mesenchymal stem cells (MSCs) from bone marrow and other tissues. Umbilical cord blood is a unique resource of stem cells and endothelial progenitor cells. Isolation of MSCs from umbilical cord blood, however, has been inefficient and inconsistent. This study was designed to examine the potential application of CD271 as a marker for the isolation of MSCs from umbilical cord blood. CD271+ cells were isolated from umbilical cord blood and bone marrow using CD271 antibody-conjugated microbeads, and characterized in osteogenic, chondrogenic and adipogenic differentiation. CD271+ cells from umbilical cord blood were slow to proliferate compared with those isolated from bone marrow. While CD271+ cells from bone marrow differentiated into osteogenic, chondrogenic and adipogenic lineages, there were no sound indications of differentiation by CD271+ cells from umbilical cord blood under the same differentiation conditions applied to the CD271+ cells from bone marrow. The study also found that bone marrow CD271+ cells remarkably upregulated the expression of chondrogenic genes under chondrogenic differentiation induction. When implanted into bone defects in mice, CD271+ cells from bone marrow regenerated significant bone, but the counterparts in umbilical cord blood formed little bone in the bone defects. In conclusion, CD271 is an efficient marker for MSC isolation from bone marrow but has failed to isolate MSCs from umbilical cord blood. CD271+ cells in bone marrow are particularly chondrogenic. The property of CD271+ cells is unique but varies from different tissues.
CD271 已被用于从骨髓和其他组织中分离间充质干细胞 (MSCs)。脐带血是干细胞和内皮祖细胞的独特资源。然而，从脐带血中分离 MSCs 的效率和一致性一直不理想。本研究旨在探讨 CD271 作为从脐带血中分离 MSCs 的标志物的潜在应用。使用 CD271 抗体偶联微珠从脐带血和骨髓中分离 CD271+细胞，并在成骨、软骨和成脂分化中进行鉴定。与从骨髓中分离的 CD271+细胞相比，从脐带血中分离的 CD271+细胞增殖缓慢。虽然骨髓来源的 CD271+细胞可分化为成骨、软骨和成脂谱系，但在相同的分化条件下，脐带血来源的 CD271+细胞没有明显的分化迹象。该研究还发现，在软骨分化诱导下，骨髓 CD271+细胞显著上调软骨基因的表达。当将骨髓 CD271+细胞植入小鼠的骨缺损中时，它们可显著再生骨，但脐带血来源的 CD271+细胞在骨缺损中形成的骨很少。总之，CD271 是从骨髓中分离 MSC 的有效标志物，但未能从脐带血中分离出 MSCs。骨髓中的 CD271+细胞特别具有成软骨特性。CD271+细胞的特性是独特的，但存在于不同的组织中有所不同。
