Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Lab Anim (NY). 2013 Jun;42(6):217-24. doi: 10.1038/laban.254.
Mammary tumors similar to those observed in women can be induced in rats by intraperitoneal administration of N-methyl-N-nitrosourea. Determining tumor volume is a useful and quantitative way to monitor tumor progression. In this study, the authors measured dimensions of rat mammary tumors using a caliper and using real-time compound B-mode ultrasonography. They then used different formulas to calculate tumor volume from these tumor measurements and compared the calculated tumor volumes with the real tumor volume to identify the formulas that gave the most accurate volume calculations. They found that caliper and ultrasonography measurements were significantly correlated but that tumor volumes calculated using different formulas varied substantially. Mammary tumors seemed to take on an oblate spheroid geometry. The most accurate volume calculations were obtained using the formula V = (W(2) × L)/2 for caliper measurements and the formula V = (4/3) × π × (L/2) × (L/2) × (D/2) for ultrasonography measurements, where V is tumor volume, W is tumor width, L is tumor length and D is tumor depth.
可以通过腹腔内给予 N-甲基-N-亚硝脲诱导大鼠产生类似于女性观察到的乳腺肿瘤。测定肿瘤体积是监测肿瘤进展的一种有用且定量的方法。在这项研究中，作者使用卡尺和实时复合 B 型超声测量大鼠乳腺肿瘤的尺寸。然后，他们使用不同的公式从这些肿瘤测量值计算肿瘤体积，并将计算出的肿瘤体积与真实肿瘤体积进行比较，以确定给出最准确体积计算的公式。他们发现，卡尺和超声测量值显著相关，但使用不同公式计算的肿瘤体积差异很大。乳腺肿瘤似乎呈扁球体几何形状。使用公式 V = (W(2) × L)/2 进行卡尺测量和公式 V = (4/3) × π × (L/2) × (L/2) × (D/2) 进行超声测量时，可获得最准确的体积计算，其中 V 是肿瘤体积，W 是肿瘤宽度，L 是肿瘤长度，D 是肿瘤深度。
