Faustino-Rocha Ana I, Gama Adelina, Oliveira Paula A, Vanderperren Katrien, Saunders Jimmy H, Pires Maria J, Ferreira Rita, Ginja Mário
Faculty of Veterinary Medicine, Lusophone University of Humanities and Technologies, Lisbon, Portugal; Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal.
Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, UTAD, 5001-911 Vila Real, Portugal; Animal and Veterinary Research Center (CECAV), School of Agrarian and Veterinary Sciences, UTAD, 5001-911 Vila Real, Portugal.
Life Sci. 2017 May 1;176:35-41. doi: 10.1016/j.lfs.2017.03.013. Epub 2017 Mar 21.
The inhibition of mast cells' degranulation may be an approach to prevent the formation of new vessels during the mammary carcinogenesis.
Female Sprague-Dawley rats were randomly divided into five experimental groups. Mammary tumors were induced by intraperitoneal injection of N-methyl-N-nitrosourea (MNU). Animals from group II were treated with ketotifen for 18weeks immediately after the MNU administration, while animals from group III only received the ketotifen after the development of the first mammary tumor. Mammary tumors vascularization was assessed by ultrasonography (Doppler, B Flow and contrast-enhanced ultrasound) and immunohistochemistry (vascular endothelial growth factor-A).
Similar to what occurs in women with breast cancer, the majority of MNU-induced mammary tumors exhibited a centripetal enhancement order of the contrast agent, clear margin and heterogeneous enhancement. Ultrasonographic and immunohistochemical data suggest that the inhibition of mast cells' degranulation did not change the mammary tumors vascularization.
抑制肥大细胞脱颗粒可能是预防乳腺癌发生过程中新血管形成的一种方法。
将雌性斯普拉格-道利大鼠随机分为五个实验组。通过腹腔注射N-甲基-N-亚硝基脲（MNU）诱导乳腺肿瘤。II组动物在给予MNU后立即用酮替芬治疗18周，而III组动物仅在第一个乳腺肿瘤发生后接受酮替芬治疗。通过超声检查（多普勒、B型血流和超声造影）和免疫组织化学（血管内皮生长因子-A）评估乳腺肿瘤血管生成情况。
与乳腺癌女性患者的情况类似，大多数MNU诱导的乳腺肿瘤表现出造影剂向心性增强顺序、边界清晰和不均匀增强。超声和免疫组织化学数据表明，抑制肥大细胞脱颗粒并未改变乳腺肿瘤的血管生成情况。
