School of Life Sciences & Biopharmaceutical Sciences, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
Biotechnol Lett. 2013 Sep;35(9):1469-73. doi: 10.1007/s10529-013-1228-0. Epub 2013 May 21.
Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in conformity with this interested enantiopreference obtained from experimental tests. The reduction of a series of other substituted aryl ketones was also investigated using the two ketoreductases.
两种来自 Candida glabrata 的酮还原酶被用于对映选择性不对称还原取代的苯乙酮，它们对取代基位于间位、对位以及邻位的卤代苯乙酮显示出不同的对映体偏好，具有优异的对映选择性。同源建模和对接分析与从实验测试中获得的这种有趣的对映体偏好相符。还使用这两种酮还原酶研究了一系列其他取代芳基酮的还原。
