A novel carbonyl reductase with anti-Prelog stereospecificity for the production of -butyl 6-cyano-(3, 5)-dihydroxyhexanoate.

A novel gene () from was cloned and then overexpressed in a recombinant strain BL21(DE3)/pET30a-crc1 of . The resulting carbonyl reductase was prepared through fermentations using the recombinant strain. The purified enzyme showed an NADPH-dependent activity and specific activity was 4.65 U/mg using -butyl 6-cyano-(5)-hydroxy-3-oxohexanoate (ATS-6) as substrate. The enzyme was optimally active at 35 °C and pH 7, respectively. The apparent and of the enzyme for ATS-6 are 1.5 mM and 21.1 μmol/min mg, respectively, indicating excellent anti-Prelog stereospecificity. Under the optimum condition, -butyl 6-cyano-(3,5)-dihydroxyhexanoate (ATS-7) was prepared with the enzyme with high value (99.9%) and good conversion (94%) in 4 h, indicating high stereoselectivity and conversion efficiency in biotransformation of ATS-6 to ATS-7.