Li Aipeng, Ye Lidan, Yang Xiaohong, Yang Chengcheng, Gu Jiali, Yu Hongwei
Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, P. R. China.
Chem Commun (Camb). 2016 May 7;52(37):6284-7. doi: 10.1039/c6cc00051g. Epub 2016 Apr 18.
The structure-guided rational design of an NADH-dependent short-chain dehydrogenase/reductase (SDR) reversed the stereoselectivity towards halogenated acetophenones from Prelog to anti-Prelog. The enzyme-substrate interactions involving an aromatic ring and a halogen atom were proven to play critical roles in determining the stereoselectivity of these ketone reductions besides the steric repulsion.
