维生素A、B1和B6缺乏对雄性大鼠草酸盐代谢影响的比较研究。

Comparative studies on the effect of vitamin A, B1 and B6 deficiency on oxalate metabolism in male rats.

作者信息

Sharma S, Sidhu H, Narula R, Thind S K, Nath R

机构信息

Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

出版信息

Ann Nutr Metab. 1990;34(2):104-11. doi: 10.1159/000177576.

DOI:10.1159/000177576

PMID:2369074

Abstract

The study was conducted to investigate the effect of vitamin A, B1 and B6 deficiency on oxalate metabolism in rats. A significant hyperoxaluria was the common observation in all the three vitamin deficiencies (vitamin B6 greater than vitamin A greater than vitamin B1). The activities of hepatic glycolate oxidase and glycolate dehydrogenase were markedly enhanced in vitamin-A- and vitamin-B6-deficient rats. However, lactate dehydrogenase levels remained unaltered in these deficiencies as compared to their respective pair-fed controls. Vitamin B1 deficiency of 4 weeks' duration could augment the activity of glycolate oxidase only, with no alterations in the glycolate dehydrogenase and lactate dehydrogenase levels. Intestinal oxalate uptake studies revealed increased bio-availability of oxalate from the gut in vitamin-A- and vitamin-B6-deficient rats. Thus, the results suggest the relative contribution of both exogenous as well as endogenous oxalate in the process of calculogenesis under various nutritional stress conditions in rat.

摘要

本研究旨在探讨维生素A、B1和B6缺乏对大鼠草酸代谢的影响。在所有三种维生素缺乏（维生素B6缺乏＞维生素A缺乏＞维生素B1缺乏）的情况下，均观察到明显的高草酸尿症。在维生素A和维生素B6缺乏的大鼠中，肝脏乙醇酸氧化酶和乙醇酸脱氢酶的活性显著增强。然而，与各自的配对喂养对照组相比，这些缺乏情况下乳酸脱氢酶水平保持不变。持续4周的维生素B1缺乏仅能增强乙醇酸氧化酶的活性，而乙醇酸脱氢酶和乳酸脱氢酶水平无变化。肠道草酸摄取研究表明，维生素A和维生素B6缺乏的大鼠肠道中草酸的生物利用度增加。因此，结果表明在大鼠各种营养应激条件下，外源性和内源性草酸在结石形成过程中都有相对贡献。