MacAulay C, Palcic B
British Columbia Cancer Research Centre, Vancouver, Canada.
Anal Quant Cytol Histol. 1990 Jun;12(3):165-71.
An automated procedure that refines the nuclear contour of a previously segmented nucleus is described. The algorithm makes use of intensity information, edge magnitude information and both object and edge connectivity information. This automated procedure generates a closed contour precisely along the edge of the nucleus. The procedure was tested on a database of 3,680 red-green-blue images of thionin-SO2 and orange II-stained cervical cells obtained from normal and dysplastic samples. When used in conjunction with a simple threshold selection algorithm and an artifact removal routine, this edge relocation algorithm resulted in the correct segmentation of over 98% of the nuclei. Only 63 (1.7%) of all nuclei were incorrectly segmented.
本文描述了一种用于细化先前分割细胞核的核轮廓的自动化程序。该算法利用强度信息、边缘幅度信息以及对象和边缘连通性信息。此自动化程序精确地沿着细胞核边缘生成闭合轮廓。该程序在一个包含3680张硫堇 - SO2和橙黄II染色的宫颈细胞红 - 绿 - 蓝图像的数据库上进行了测试，这些图像取自正常和发育异常样本。当与简单的阈值选择算法和伪像去除程序结合使用时，这种边缘重定位算法导致超过98%的细胞核被正确分割。所有细胞核中只有63个（1.7%）被错误分割。
