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[过氧化氢存在下人体血清鲁米诺依赖性化学发光的机制]

[A mechanism of luminol-dependent chemiluminescence of human serum in the presence of hydrogen peroxide].

作者信息

Kozlov A V, Osipov A N, Vladimirov Iu A

出版信息

Biofizika. 1990 Mar-Apr;35(2):347-9.

PMID:2369607
Abstract

Comparative role of proteins of the human blood plasma in luminol-dependent induction of chemiluminescence in the presence of hydrogen peroxide was studied. It was found that the largest contribution into chemiluminescence was made by the plasma fraction with the molecular mass 250 kD. Besides the intensity of chemiluminescence proves to be proportional to hemoglobin concentration, which when added to the blood plasma entered the fractions with the molecular mass about 250 kD. It is suggested that hemoglobin producing luminescence in the blood plasma is related to haptoglobin.

摘要

研究了人血浆蛋白在过氧化氢存在下对鲁米诺依赖性化学发光诱导中的比较作用。发现分子量为250 kD的血浆组分对化学发光的贡献最大。此外,化学发光强度被证明与血红蛋白浓度成正比,当血红蛋白添加到血浆中时,它进入分子量约为250 kD的组分中。有人提出,血浆中产生发光的血红蛋白与触珠蛋白有关。

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