Ning J, Chang T M
Artificial Cells and Organs Research Centre, McGill University, Montreal, Canada.
Biomater Artif Cells Artif Organs. 1990;18(2):203-17. doi: 10.3109/10731199009117302.
We developed a method to determine whole complement hemolytic activity (CH50) in rat plasma using a modification of the method of Mayer(1). Plasma CH50 level in 30 male Sprague Dawley rats was 1000 +/- 200 units. Following infusions with human and rat stroma-free hemoglobin or polyhemoglobin solutions, CH50 did not change significantly in rats when compared to albumin infused rats. On the other hand, CH50 significantly decreased in the group receiving membrane stroma and bacterial endotoxins.
我们采用对迈耶（1）的方法进行改良的方式，开发了一种测定大鼠血浆中总补体溶血活性（CH50）的方法。30只雄性斯普拉格-道利大鼠的血浆CH50水平为1000±200单位。在输注人源和大鼠无基质血红蛋白或多血红蛋白溶液后，与输注白蛋白的大鼠相比，大鼠的CH50没有显著变化。另一方面，接受膜基质和细菌内毒素的组中CH50显著降低。
