Ning J, Chang T M
Artificial Cells and Organs Research Centre, McGill University, Montreal, Canada.
Int J Artif Organs. 1990 Aug;13(8):509-16.
We studied the effects of rat stroma-free hemoglobin (rSFH), human stroma-free hemoglobin (hSFH), rat polyhemoglobin (rPoly), and human polyhemoglobin (hPoly) on coagulation factors in rats. Albumin and saline infused rats were controls. The infusion volume was 10% of the rat's blood volume. The concentrations of hemoglobin in this study were 7 g/dl. Measurements for prothrombin time (PT) and activated partial thromboplastin time (PTT) were at 5 minutes, 2, 6, 24 and 72 hours after infusion. Factor X, fibrinogen, plasminogen, antithrombin III, and antiplasmin were followed at 24 and 72 hours after infusion. Compared with saline infused rats PT and PTT did not change significantly in those rats infused with Hb preparations. There was a transient increase of PTT from 2 to 24 hours after infusion in albumin infused rats. Factor X, fibrinogen, antithrombin III and antiplasmin showed no significant differences between Hb infused groups and saline infused group. Twenty-four hours and 72 hours after infusion plasminogen decreased in all groups except the albumin infused rats at 24 hours after infusion when compared with normal rat plasma pool. However, there were no significant differences in plasminogen levels between the hemoglobin infused groups and the control saline group. Stroma-free and polyHb solutions (rSFH, hSFH, rPoly and hPoly) did not cause significant changes in prothrombin time and activated partial thromboplastin time in rats. The rats infused with hemoglobin solutions (rSFH, hSFH, rPoly, and hPoly) did not show significant differences in Factor X, fibrinogen, antithrombin III and antiplasmin levels compared with the control group.
我们研究了大鼠无基质血红蛋白（rSFH）、人无基质血红蛋白（hSFH）、大鼠多聚血红蛋白（rPoly）和人多聚血红蛋白（hPoly）对大鼠凝血因子的影响。输注白蛋白和生理盐水的大鼠作为对照。输注量为大鼠血容量的10%。本研究中血红蛋白浓度为7 g/dl。在输注后5分钟、2小时、6小时、24小时和72小时测量凝血酶原时间（PT）和活化部分凝血活酶时间（PTT）。在输注后24小时和72小时追踪因子X、纤维蛋白原、纤溶酶原、抗凝血酶III和抗纤溶酶。与输注生理盐水的大鼠相比，输注血红蛋白制剂的大鼠PT和PTT没有显著变化。输注白蛋白的大鼠在输注后2至24小时PTT有短暂升高。因子X、纤维蛋白原、抗凝血酶III和抗纤溶酶在输注血红蛋白组和输注生理盐水组之间没有显著差异。与正常大鼠血浆池相比，输注后24小时和72小时，除输注白蛋白的大鼠在输注后24小时外，所有组的纤溶酶原均下降。然而，输注血红蛋白组和对照生理盐水组之间纤溶酶原水平没有显著差异。无基质和多聚血红蛋白溶液（rSFH、hSFH、rPoly和hPoly）不会引起大鼠凝血酶原时间和活化部分凝血活酶时间的显著变化。与对照组相比，输注血红蛋白溶液（rSFH、hSFH、rPoly和hPoly）的大鼠在因子X、纤维蛋白原、抗凝血酶III和抗纤溶酶水平上没有显著差异。
