Ning J, Chang T M
Artificial Cells and Organs Research Centre, McGill University, Montreal, Canada.
Biomater Artif Cells Artif Organs. 1990;18(2):219-32. doi: 10.3109/10731199009117303.
This is a study of the effects of stroma-free hemoglobin(Hb) and polyhemoglobin on C3 and C3a levels and on blood cell counts. The effects of membrane stroma from red cells and endotoxins on complement activation were also investigated. Plasma samples from rats were incubated with hemoglobin solutions or control solutions. C3 was measured using anti-rat C3 by nephelometric method. C3a was measured using radioimmunoassay. C3 and C3a were also determined in rats receiving hemoglobin solutions, red cell stroma solutions, or a solution containing complement activators (membrane stroma and bacterial endotoxins). There were no significant differences in C3 or C3a levels between the plasma incubated with hemoglobin solutions and the control plasma incubated with saline (P greater than 0.05). Compared with the saline incubated plasma, C3 was significantly lower and C3a significantly higher in the zymosan treated plasma (P less than 0.01). In hemoglobin infused rats, there was no significant difference between pre-versus post-infusion values in C3 and C3a. There was a significant increase in C3a in the rats receiving stroma suspension (P less than 0.05) or stroma and endotoxins (P less than 0.02). Polyhemoglobin and stroma-free hemoglobin did not cause significant changes in total leucocyte, differential, or platelet counts. Our study suggests that purified stroma-free Hb and polyHb do not activate complement, and that C3a is a more sensitive parameter for monitoring complement activation than C3. On the other hand, contaminates in hemoglobin preparations, such as membrane stroma or endotoxins, activate the complement system.
这是一项关于无基质血红蛋白（Hb）和多聚血红蛋白对C3及C3a水平以及血细胞计数影响的研究。同时还研究了红细胞膜基质和内毒素对补体激活的影响。将大鼠的血浆样本与血红蛋白溶液或对照溶液进行孵育。采用比浊法使用抗大鼠C3检测C3。采用放射免疫分析法检测C3a。还在接受血红蛋白溶液、红细胞基质溶液或含有补体激活剂（膜基质和细菌内毒素）溶液的大鼠中测定C3和C3a。与用生理盐水孵育的对照血浆相比，用血红蛋白溶液孵育的血浆中C3或C3a水平无显著差异（P大于0.05）。与用生理盐水孵育的血浆相比，酵母聚糖处理的血浆中C3显著降低，C3a显著升高（P小于0.01）。在输注血红蛋白的大鼠中，输注前后C3和C3a的值无显著差异。接受基质悬液（P小于0.05）或基质与内毒素（P小于0.02）的大鼠中C3a显著升高。多聚血红蛋白和无基质血红蛋白不会引起白细胞总数、分类或血小板计数的显著变化。我们的研究表明，纯化的无基质Hb和多聚Hb不会激活补体，并且C3a是比C3更敏感的监测补体激活的参数。另一方面，血红蛋白制剂中的污染物，如膜基质或内毒素，会激活补体系统。
