Ruan Sen-Lin, Wang Ru, Chen Xiao-Yan, Wu Ya-Ying, Liu Qing, Liu Min, Rao Cuip, Deng Hong
Department of Pathology, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 Feb;33(2):354-8.
In the present paper, lyophilized sera from 31 patients with colorectal cancer, 8 patients with enteritis and 10 healthy controls were measured using Nicolet Nexus-670 Fourier Transform Infrared (FTIR) spectrometer, and the spectral features were investigated and comparison among three groups was made. The result showed that (1) there are 11 peaks of absorption in the spectrum of lyophilized sera, the wavenumber of the ninth peak moved to 1 249 cm(-1) in the colorectal cancer group and significant difference was observed with the control group (Z = -2.051, P < 0.05); Five ratios of absorbance were calculated and no significant difference was observed among three groups; (2) spectrum in the region of 1 700-1 000 cm(-1) was analysed with the method of hierarchical cluster analysis and showed only partial success in distinguishing the three groups; (2) spectrum of amide I peptide (1 700-1 600 cm(-1)) was deconvolved to get the percentage composition of protein secondary structures, and no significant difference was observed among three groups. The results provide a preliminary theoretical basic for using FTIR spectrum of lyophilized serum for the early diagnosis of colorectal cancer.
在本研究中，使用尼高力Nexus - 670傅里叶变换红外（FTIR）光谱仪对31例结直肠癌患者、8例肠炎患者的冻干血清以及10名健康对照者的血清进行了检测，并对光谱特征进行了研究，同时对三组进行了比较。结果显示：（1）冻干血清光谱中有11个吸收峰，结直肠癌组第九个峰的波数移至1249 cm⁻¹，与对照组相比差异有统计学意义（Z = -2.051，P < 0.05）；计算了五个吸光度比值，三组间差异无统计学意义；（2）采用层次聚类分析法对1700 - 1000 cm⁻¹区域的光谱进行分析，在区分三组方面仅取得部分成功；（2）对酰胺I肽（1700 - 1600 cm⁻¹）的光谱进行去卷积以获得蛋白质二级结构的百分比组成，三组间差异无统计学意义。这些结果为利用冻干血清的FTIR光谱进行结直肠癌的早期诊断提供了初步的理论基础。
