Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Department of General Surgery, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Mar 25;122:288-94. doi: 10.1016/j.saa.2013.11.031. Epub 2013 Nov 16.
The aim of this study is to confirm FTIR spectroscopy as a diagnostic tool for colorectal cancer. 180 freshly removed colorectal samples were collected from 90 patients for spectrum analysis. The ratios of spectral intensity and relative intensity (/I1460) were calculated. Principal component analysis (PCA) and Fisher's discriminant analysis (FDA) were applied to distinguish the malignant from normal. The FTIR parameters of colorectal cancer and normal tissues were distinguished due to the contents or configurations of nucleic acids, proteins, lipids and carbohydrates. Related to nitrogen containing, water, protein and nucleic acid were increased significantly in the malignant group. Six parameters were selected as independent factors to perform discriminant functions. The sensitivity for FTIR in diagnosing colorectal cancer was 96.6% by discriminant analysis. Our study demonstrates that FTIR can be a useful technique for detection of colorectal cancer and may be applied in clinical colorectal cancer diagnosis.
本研究旨在证实傅里叶变换红外（FTIR）光谱分析可作为结直肠癌的一种诊断工具。从 90 例患者中采集了 180 例新鲜切除的结直肠样本进行光谱分析。计算光谱强度比和相对强度（/I1460）。应用主成分分析（PCA）和 Fisher 判别分析（FDA）来区分良恶性。由于核酸、蛋白质、脂类和碳水化合物的含量或结构不同，结直肠癌和正常组织的 FTIR 参数有所不同。与含氮、水、蛋白质和核酸相关的参数在恶性组中显著增加。选择 6 个参数作为独立因素进行判别函数分析。判别分析中 FTIR 诊断结直肠癌的灵敏度为 96.6%。本研究表明，FTIR 可作为结直肠癌检测的一种有用技术，有望应用于临床结直肠癌的诊断。
