Chen R B, Suzuki K, Nomura T, Nakajima T
Dental Clinic, Ojiya General Hospital, Japan.
J Oral Maxillofac Surg. 1993 Apr;51(4):397-401. doi: 10.1016/s0278-2391(10)80354-4.
Nuclear DNA content and cell kinetics were studied in 40 squamous cell carcinomas of the oral cavity by flow cytometry to evaluate the diagnostic significance of the method in predicting lymph node metastasis. The presence of lymph node metastasis was confirmed histologically in 20 carcinomas. The incidence of metastasis was 36% in group A (22 carcinomas of the tongue and floor of the mouth) and 67% in group B (18 carcinomas of the alveolus and gingiva). On flow cytometric analysis, DNA aneuploidy was observed in 23 tumors (58%). The incidence of lymph node metastasis in the aneuploid tumors was 70%, which was significantly higher than the 24% for the diploid tumors. This also was the case for both of the subgroups. The incidence of aneuploidy and the DNA index for tumors with metastasis were 80% and 1.58, respectively. These values were significantly higher than the 35% and 1.16 for tumors without metastasis, but there were no significant differences in the S phase and G2M phase fractions between the tumors with and without metastasis. The incidence of aneuploidy in tumors with metastasis was also higher in group A (75% versus 36%) and group B (83% versus 33%). In terms of histologic differentiation, the incidence of metastasis and aneuploidy increased as the degree of differentiation decreased. Similar relationships were found between the histologic grade of malignancy and the incidence of metastasis and aneuploidy. The results indicate that nuclear DNA content analysis by flow cytometry is useful as a supplement to clinical and histologic evaluation in predicting the tendency of squamous cell carcinomas of the oral cavity to metastasize to regional lymph nodes.
通过流式细胞术研究了40例口腔鳞状细胞癌的核DNA含量和细胞动力学，以评估该方法在预测淋巴结转移方面的诊断意义。经组织学证实，20例癌存在淋巴结转移。A组（22例舌癌和口底癌）转移发生率为36%，B组（18例牙槽突癌和牙龈癌）转移发生率为67%。流式细胞术分析显示，23例肿瘤（58%）存在DNA非整倍体。非整倍体肿瘤的淋巴结转移发生率为70%，显著高于二倍体肿瘤的24%。两个亚组情况均如此。有转移肿瘤的非整倍体发生率和DNA指数分别为80%和1.58。这些值显著高于无转移肿瘤的35%和1.16，但有转移和无转移肿瘤之间的S期和G2M期比例无显著差异。A组（75%对36%）和B组（83%对33%）中有转移肿瘤的非整倍体发生率也更高。在组织学分化方面，转移和非整倍体发生率随分化程度降低而增加。在恶性组织学分级与转移和非整倍体发生率之间也发现了类似关系。结果表明，通过流式细胞术分析核DNA含量有助于补充临床和组织学评估，以预测口腔鳞状细胞癌向区域淋巴结转移的倾向。
