血清和条件培养基对细胞铺展的刺激和抑制作用。

Stimulatory and inhibitory effects of serum and conditioned medium on cell spreading.

作者信息

Verger C, Imbenotte J

机构信息

C.N.R.S., UPR 303 I.R.S.C., Villejuif, France.

出版信息

Cell Biol Int Rep. 1990 Jun;14(6):543-8. doi: 10.1016/0309-1651(90)91181-3.

DOI:10.1016/0309-1651(90)91181-3

PMID:2369762

Abstract

This work deals with the effects of coating culture dishes with chick embryo fibroblast conditioned medium (CM) and 1% fetal calf serum (FCS) on the rates of attachment and spreading of chick embryo fibroblasts. It appeared that a FCS coating over a CM precoating exerted a remarkable promoting cooperative effect on cell spreading whereas a CM coating over a FCS precoating had a very marked inhibitory effect. Precoating with cobalt-protoporphyrin IX (CoPP) was also performed to serve as a substrate for FCS or CM coating. This CoPP precoating was found to exert a promoting effect for FCS coating only.

摘要

这项工作研究了用鸡胚成纤维细胞条件培养基（CM）和1%胎牛血清（FCS）包被培养皿对鸡胚成纤维细胞附着和铺展速率的影响。结果表明，在CM预包被上覆盖FCS对细胞铺展具有显著的促进协同作用，而在FCS预包被上覆盖CM则具有非常显著的抑制作用。还用钴原卟啉IX（CoPP）进行预包被，作为FCS或CM包被的底物。发现这种CoPP预包被仅对FCS包被有促进作用。