Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
Nutrients. 2013 May 21;5(5):1660-71. doi: 10.3390/nu5051660.
Uncoupling protein 3 (UCP3) is a mitochondrial membrane transporter that is expressed mainly in skeletal muscle where it plays an important role in energy expenditure and fat oxidation. In this study, we investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on UCP3 gene expression in C(2)C(12) muscle cells. EPA and DHA up-regulated UCP3 mRNA level in a dose-dependent manner and similarly increased UCP3 promoter activity in C(2)C(12) muscle cells. To determine whether AMP-activated protein kinase (AMPK) signaling may also directly regulate UCP3 expression, 5'-amino-4-imidazolecarboxamide-ribonucleoside (AICAR), an AMP analog that activates AMPK, was treated in C(2)C(12) muscle cells. AICAR showed additive effects with EPA or DHA on the UCP3 promoter activation. These results indicate that EPA and DHA directly regulate the gene expression of UCP3, potentially through AMPK-mediated pathway in C(2)C(12) muscle cells.
解偶联蛋白 3（UCP3）是一种线粒体膜转运蛋白，主要在骨骼肌中表达，在能量消耗和脂肪氧化中发挥重要作用。在这项研究中，我们研究了二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）对 C（2）C（12）肌肉细胞中 UCP3 基因表达的影响。EPA 和 DHA 呈剂量依赖性地上调 UCP3 mRNA 水平，并在 C（2）C（12）肌肉细胞中类似地增加 UCP3 启动子活性。为了确定 AMP 激活的蛋白激酶（AMPK）信号是否也可以直接调节 UCP3 的表达，5'-氨基-4-咪唑甲酰胺-核糖核苷酸（AICAR），一种激活 AMPK 的 AMP 类似物，在 C（2）C（12）肌肉细胞中进行处理。AICAR 与 EPA 或 DHA 一起对 UCP3 启动子的激活具有相加作用。这些结果表明，EPA 和 DHA 直接调节 UCP3 的基因表达，可能通过 C（2）C（12）肌肉细胞中的 AMPK 介导途径。
