Chaudhry M Ahmad
Department of Medical Laboratory and Radiation Sciences, University of Vermont, Burlington, VT 05405, USA.
Int J Mol Sci. 2013 Apr 25;14(5):9099-110. doi: 10.3390/ijms14059099.
A wide variety of biological effects are induced in cells that are exposed to ionizing radiation. The expression changes of coding mRNA and non-coding micro-RNA have been implicated in irradiated cells. The involvement of other classes of non-coding RNAs (ncRNA), such as small nucleolar RNAs (snoRNAs), long ncRNAs (lncRNAs), and PIWI-interacting RNAs (piRNAs) in cells recovering from radiation-induced damage has not been examined. Thus, we investigated whether these ncRNA were undergoing changes in cells exposed to ionizing radiation. The modulation of ncRNAs expression was determined in human TK6 (p53 positive) and WTK1 (p53 negative) cells. The snoRNA host genes SNHG1, SNHG6, and SNHG11 were induced in TK6 cells. In WTK1 cells, SNHG1 was induced but SNHG6, and SNHG11 were repressed. SNHG7 was repressed in TK6 cells and was upregulated in WTK1 cells. The lncRNA MALAT1 and SOX2OT were induced in both TK6 and WTK1 cells and SRA1 was induced in TK6 cells only. Interestingly, the MIAT and PIWIL1 were not expressed in TK6 cells before or after the ionizing radiation treatment. The MIAT and PIWIL1 were upregulated in WTK1 cells. This data provides evidence that altered ncRNA expression is a part of the complex stress response operating in radiation-treated cells and this response depends on functional p53.
暴露于电离辐射的细胞会产生各种各样的生物学效应。编码mRNA和非编码微小RNA的表达变化与受辐照细胞有关。其他种类的非编码RNA（ncRNA），如小核仁RNA（snoRNA）、长链ncRNA（lncRNA）和PIWI相互作用RNA（piRNA）在从辐射诱导损伤中恢复的细胞中的作用尚未得到研究。因此，我们研究了这些ncRNA在暴露于电离辐射的细胞中是否发生变化。在人TK6（p53阳性）和WTK1（p53阴性）细胞中测定了ncRNA表达的调节情况。snoRNA宿主基因SNHG1、SNHG6和SNHG11在TK6细胞中被诱导。在WTK1细胞中，SNHG1被诱导，但SNHG6和SNHG11被抑制。SNHG7在TK6细胞中被抑制，在WTK1细胞中上调。lncRNA MALAT1和SOX2OT在TK6和WTK1细胞中均被诱导，而SRA1仅在TK6细胞中被诱导。有趣的是，MIAT和PIWIL1在电离辐射处理前后的TK6细胞中均未表达。MIAT和PIWIL1在WTK1细胞中上调。这些数据证明，ncRNA表达的改变是辐射处理细胞中复杂应激反应的一部分，并且这种反应取决于功能性p53。
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