Liu Jing, Zhou Feng, Li Guang-Yong, Wang Lin, Li Hui-Xi, Bai Guang-Yi, Guan Rui-Li, Xu Yong-De, Gao Ze-Zhu, Tian Wen-Jie, Xin Zhong-Cheng
Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
Int J Mol Sci. 2013 May 21;14(5):10661-73. doi: 10.3390/ijms140510661.
To investigate the therapeutic effect of different doses of low energy shock wave therapy (LESWT) on the erectile dysfunction (ED) in streptozotocin (STZ) induced diabetic rats. SD rats (n = 75) were randomly divided into 5 groups (normal control, diabetic control, 3 different dose LESWT treated diabetic groups). Diabetic rats were induced by intra-peritoneal injection of STZ (60 mg/kg) and rats with fasting blood glucose ≥ 300 mg/dL were selected as diabetic models. Twelve weeks later, different doses of LESWT (100, 200 and 300 shocks each time) treatment on penises were used to treat ED (7.33 MPa, 2 shocks/s) three times a week for two weeks. The erectile function was evaluated by intracavernous pressure (ICP) after 1 week washout period. Then the penises were harvested for histological study. The results showed LESWT could significantly improve the erectile function of diabetic rats, increase smooth muscle and endothelial contents, up-regulate the expression of α-SMA, vWF, nNOS and VEGF, and down- regulate the expression of RAGE in corpus cavernosum. The therapeutic effect might relate to treatment dose positively, and the maximal therapeutic effect was noted in the LESWT300 group. Consequently, 300 shocks each time might be the ideal LESWT dose for diabetic ED treatment.
为研究不同剂量低能量冲击波疗法（LESWT）对链脲佐菌素（STZ）诱导的糖尿病大鼠勃起功能障碍（ED）的治疗效果。将75只SD大鼠随机分为5组（正常对照组、糖尿病对照组、3个不同剂量LESWT治疗的糖尿病组）。通过腹腔注射STZ（60 mg/kg）诱导糖尿病大鼠，选取空腹血糖≥300 mg/dL的大鼠作为糖尿病模型。12周后，采用不同剂量的LESWT（每次100、200和300次冲击）对阴茎进行治疗ED（7.33 MPa，每秒2次冲击），每周3次，共2周。在1周洗脱期后，通过海绵体内压（ICP）评估勃起功能。然后摘取阴茎进行组织学研究。结果显示，LESWT可显著改善糖尿病大鼠的勃起功能，增加平滑肌和内皮细胞含量，上调海绵体内α-SMA、vWF、nNOS和VEGF的表达，并下调RAGE的表达。治疗效果可能与治疗剂量呈正相关，在LESWT300组观察到最大治疗效果。因此，每次300次冲击可能是糖尿病ED治疗的理想LESWT剂量。
