Schaumann B A, Winge V B, Pederson M, Kuskowski M A
Research Service, Veterans Administration Medical Center, Portland, OR 97207.
Epilepsia. 1990 Jul-Aug;31(4):453-7. doi: 10.1111/j.1528-1157.1990.tb05502.x.
The potential of the widely prescribed antiepileptic drugs (AEDs) phenytoin (PHT) and phenobarbital (PB) to interact with genetic material was tested using sister chromatid exchange (SCE) assay. Thirty adult male patients with epilepsy receiving long-term AED therapy (16 with PHT, 6 with PB, and 8 with combined PHT and PB therapy) and 30 healthy controls were selected for the study of SCE frequencies in peripheral lymphocytes. The patients and controls were carefully screened and matched for sex, age, and smoking habits. All potential probands with exposure to factors known or suspected of affecting the SCE frequencies were excluded. Statistical analyses did not show any significant differences between the SCE rates of PHT- and/or PB-treated patients and controls, indicating a lack of mutagenicity of the tested drugs as expressed by induction of SCE on adult recipients. Smoking, however, affected the SCE levels considerably. The smokers had higher SCE frequencies than the nonsmokers, both among patients and controls. Caffeine consumption was also associated with SCE increases in patients but not in controls.
使用姐妹染色单体交换（SCE）试验检测了广泛使用的抗癫痫药物（AEDs）苯妥英（PHT）和苯巴比妥（PB）与遗传物质相互作用的可能性。选取了30例接受长期AED治疗的成年男性癫痫患者（16例使用PHT，6例使用PB，8例使用PHT和PB联合治疗）以及30名健康对照者，研究外周血淋巴细胞中的SCE频率。对患者和对照者进行了仔细筛选，并在性别、年龄和吸烟习惯方面进行了匹配。排除了所有接触已知或疑似影响SCE频率因素的潜在先证者。统计分析未显示PHT和/或PB治疗患者与对照者的SCE率有任何显著差异，表明受试药物对成年接受者未表现出通过诱导SCE所表达的致突变性。然而，吸烟对SCE水平有相当大的影响。无论是在患者还是对照者中，吸烟者的SCE频率均高于不吸烟者。咖啡因摄入也与患者的SCE增加有关，但与对照者无关。
