Hofer M, Pagliusi S R, Hohn A, Leibrock J, Barde Y A
Max-Planck Institute for Psychiatry, Department of Neurochemistry, Martinsried, FRG.
EMBO J. 1990 Aug;9(8):2459-64. doi: 10.1002/j.1460-2075.1990.tb07423.x.
Brain-derived neurotrophic factor (BDNF) is a protein that allows the survival of specific neuronal populations. This study reports on the distribution of the BDNF mRNA in the adult mouse brain, where the BDNF gene is strongly expressed, using quantitative Northern blot analysis and in situ hybridization. All brain regions examined were found to contain substantial amounts of BDNF mRNA, the highest levels being found in the hippocampus followed by the cerebral cortex. In the hippocampus, which is also the site of highest nerve growth factor (NGF) gene expression in the central nervous system (CNS), there is approximately 50-fold more BDNF mRNA than NGF mRNA. In other brain regions, such as the granule cell layer of the cerebellum, the differences between the levels of BDNF and NGF mRNAs are even more pronounced. The BDNF mRNA was localized by in situ hybridization in hippocampal neurons (pyramidal and granule cells). These data suggest that BDNF may play an important role in the CNS for a wide variety of adult neurons.
脑源性神经营养因子（BDNF）是一种能使特定神经元群体存活的蛋白质。本研究采用定量Northern印迹分析和原位杂交技术，报道了成年小鼠脑中BDNF基因强烈表达时BDNF mRNA的分布情况。研究发现，所有检测的脑区都含有大量的BDNF mRNA，其中海马体中的含量最高，其次是大脑皮层。在海马体中，它也是中枢神经系统（CNS）中神经生长因子（NGF）基因表达最高的部位，BDNF mRNA的含量大约是NGF mRNA的50倍。在其他脑区，如小脑颗粒细胞层，BDNF和NGF mRNA水平的差异更为明显。通过原位杂交在海马神经元（锥体细胞和颗粒细胞）中定位了BDNF mRNA。这些数据表明，BDNF可能在中枢神经系统中对多种成年神经元发挥重要作用。
