Tanaka Koji, Kawamura Mikio, Otake Kohei, Toiyama Yuji, Okugawa Yoshinaga, Inoue Yasuhiro, Uchida Keiichi, Araki Toshimitsu, Mohri Yasuhiko, Kusunoki Masato
Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan,
Surg Today. 2014 Feb;44(2):332-9. doi: 10.1007/s00595-013-0625-2. Epub 2013 May 23.
Trehalose, naturally occurring disaccharide, has been reported to prevent postoperative abdominal adhesions in animal models. We investigated whether trehalose affects the function of human polymorphonuclear neutrophils (PMNs) in vitro to assess the feasibility of its clinical application as an anti-adhesive barrier.
Human PMNs were obtained from 17 healthy volunteers. Escherichia coli and Staphylococcus aureus were used for the bacterial infection model, whereas lipopolysaccharide (LPS) and interleukin (IL)-1β were used for inflammation induction model. The PMN phagocytosis rates of bacteria and apoptosis/necrosis were assessed on trehalose, maltose, and control media. Cytokines; namely, tumor necrosis factor-α, IL-1α, IL-1Ra, IL-6, and IL-8; and PMN-elastase were measured on each medium in both models.
There were no significant differences in the phagocytosis rates, apoptosis/necrosis rates, or levels of all cytokines or PMN-elastase among the three media in the bacterial infection model. There were also no significant differences in the levels of all cytokines and PMN-elastase among the three media in the IL-1β inflammation induction model. PMN-elastase was lower in trehalose and maltose medium after LPS stimulation, at 3 and 24 h.
Our results suggest that trehalose does not affect the cellular function, cytokine production, or release of PMN-elastase of human PMNs in an in vitro bacterial infection model.
海藻糖是一种天然存在的二糖，据报道在动物模型中可预防术后腹腔粘连。我们研究了海藻糖是否会在体外影响人多形核中性粒细胞（PMN）的功能，以评估其作为抗粘连屏障临床应用的可行性。
从17名健康志愿者身上获取人PMN。使用大肠杆菌和金黄色葡萄球菌建立细菌感染模型，而使用脂多糖（LPS）和白细胞介素（IL）-1β建立炎症诱导模型。在海藻糖、麦芽糖和对照培养基上评估PMN对细菌的吞噬率以及凋亡/坏死情况。在两种模型的每种培养基上检测细胞因子，即肿瘤坏死因子-α、IL-1α、IL-1Ra、IL-6和IL-8，以及PMN弹性蛋白酶。
在细菌感染模型中，三种培养基之间的吞噬率、凋亡/坏死率或所有细胞因子及PMN弹性蛋白酶水平均无显著差异。在IL-1β炎症诱导模型中，三种培养基之间所有细胞因子及PMN弹性蛋白酶水平也无显著差异。LPS刺激后3小时和24小时，海藻糖和麦芽糖培养基中的PMN弹性蛋白酶水平较低。
我们的结果表明，在体外细菌感染模型中，海藻糖不会影响人PMN的细胞功能、细胞因子产生或PMN弹性蛋白酶的释放。
