Department of Respiratory and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang, 100020, Beijing, China.
Lung. 2013 Aug;191(4):385-9. doi: 10.1007/s00408-013-9474-4. Epub 2013 May 23.
Our previous data have demonstrated that the number of IL-9-producing CD4(+) T cells (Th9 cells) in malignant pleural effusion (MPE) was significantly increased when compared with that in blood. The aim of the present study was to investigate the mechanism by which Th9 cells were recruited into MPE and the phenotypic characteristics of pleural Th9 cells.
The expression patterns of chemokine receptors (CCRs) on Th9 cells and the chemoattractant activity of chemokine CCL20 for Th9 cells in vitro were observed. The phenotypic features of Th9 cells in MPE were determined by flow cytometry.
We found that Th9 cells in both MPE and blood expressed a high level of CCR6 on their surface. An in vitro migration assay confirmed that both MPE and supernatants of cultured pleural mesothelial cells could induce the migration of Th9 cells, and anti-CCL20 mAb significantly inhibited the ability of MPE or supernatants to stimulate Th9 cell chemotaxis. We also noted that pleural Th9 cells expressed high levels of CD45RO and very low levels of CD45RA and CD62L, displaying the phenotype of effector memory cells.
Our data revealed that recruitment of Th9 cells into MPE could be induced by pleural CCL20 and that the majority of Th9 cells in MPE displayed the phenotype of effector memory cells.
我们之前的数据表明，与血液相比，恶性胸腔积液（MPE）中产生白细胞介素-9（IL-9）的 CD4+T 细胞（Th9 细胞）数量显著增加。本研究旨在探讨 Th9 细胞被招募到 MPE 中的机制以及胸膜 Th9 细胞的表型特征。
观察 Th9 细胞上趋化因子受体（CCR）的表达模式和趋化因子 CCL20 对 Th9 细胞的体外趋化活性。通过流式细胞术确定 MPE 中 Th9 细胞的表型特征。
我们发现，MPE 和血液中的 Th9 细胞表面均高表达 CCR6。体外迁移实验证实，MPE 和培养的胸膜间皮细胞上清液均可诱导 Th9 细胞迁移，抗 CCL20 mAb 可显著抑制 MPE 或上清液刺激 Th9 细胞趋化的能力。我们还注意到，胸膜 Th9 细胞表达高水平的 CD45RO，极低水平的 CD45RA 和 CD62L，呈现效应记忆细胞的表型。
我们的数据表明，Th9 细胞被招募到 MPE 中可被胸膜 CCL20 诱导，并且 MPE 中的大多数 Th9 细胞呈现效应记忆细胞的表型。
