Department of Otorhinolaryngology, Lithuanian University of Health Sciences (LUHS), LT-50161 Kaunas, Lithuania.
Neuroscience Institute, Lithuanian University of Health Sciences (LUHS), LT-50161 Kaunas, Lithuania.
Cells. 2021 Mar 9;10(3):601. doi: 10.3390/cells10030601.
Recent studies have described the dichotomous function of IL-9 in various cancer diseases. However, its function has still not been analysed in laryngeal squamous cell carcinoma (LSCC). In the present study, we evaluated five single nucleotide polymorphisms (SNPs) of (rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884) and determined their associations with the patients' five-year survival rate. Additionally, we analysed serum IL-9 levels using an enzyme-linked immunosorbent assay. Three hundred LSCC patients and 533 control subjects were included in this study. A significant association between the patients' survival rate and distribution of rs1859430 variants was revealed: patients carrying AA genotype had a higher risk of dying ( = 0.005). Haplotypes A-G-C-G-G of (rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884) were associated with 47% lower odds of LSCC occurrence ( = 0.035). Serum IL-9 levels were found detectable in three control group subjects (8.99 ± 12.03 pg/mL). In summary, these findings indicate that the genotypic distribution of rs1859430 negatively influences the five-year survival rate of LSCC patients. The haplotypes A-G-C-G-G of (rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884) are associated with the lower odds of LSCC development.
最近的研究描述了白细胞介素-9 (IL-9) 在各种癌症疾病中的双重功能。然而，它在喉鳞状细胞癌 (LSCC) 中的功能尚未得到分析。在本研究中，我们评估了 (rs1859430、rs2069870、rs11741137、rs2069885 和 rs2069884) 的五个单核苷酸多态性 (SNP)，并分析了它们与患者五年生存率的关系。此外，我们还使用酶联免疫吸附试验分析了血清 IL-9 水平。本研究纳入了 300 例 LSCC 患者和 533 例对照者。结果显示，患者生存率与 rs1859430 变异体的分布存在显著相关性：携带 AA 基因型的患者死亡风险更高( = 0.005)。 (rs1859430、rs2069870、rs11741137、rs2069885 和 rs2069884) 的 A-G-C-G-G 单倍型与 LSCC 发生的几率降低 47%相关( = 0.035)。在 3 名对照组受试者中检测到可检测的血清 IL-9 水平(8.99 ± 12.03 pg/mL)。综上所述，这些发现表明， rs1859430 的基因型分布对 LSCC 患者的五年生存率有负面影响。 (rs1859430、rs2069870、rs11741137、rs2069885 和 rs2069884) 的 A-G-C-G-G 单倍型与 LSCC 发病几率降低相关。
