Zheng Yi-Qun, Li Zhi-Jian, Gao Xin, Zhang Xiao-Bin, Li Fa-Jiang, Shi Ying-Jiang, Luo Gang, Li Wei
Department of Urology, Zhongshan Hospital Affiliated to Sun Yat-sen University, Zhongshan, Guangdong 528403, China.
Zhonghua Nan Ke Xue. 2013 Mar;19(3):205-9.
To investigate the expression of the Eag1 K( +) channel in the prostate cancer (PCa) tissue, its correlation with the development and progression of PCa, and whether it could be a target for the diagnosis and treatment of PCa.
We used RT-PCR and immunohistochemistry to determine the mRNA and protein expressions of the Eag1 K(+) channel in the normal peritumoral tissue of androgen-dependent PCa (ADPCa) (group A) and androgen-independent PCa (AIPCa) (group B) as well as in the tumorous tissue of ADPCa (group C) and AIPCa (group D).
The relative coefficients of the mRNA expression of the Eag1 K(+) channel were 0.265 +/- 0.413, 0.167 +/- 0.511, 2.673 +/- 2.988 and 2.815 +/- 2.901 in groups A, B, C and D, respectively, increased significantly in the latter two groups (P < 0.05). The positive rates of the protein expression of the Eag1 K (+) channel were significantly higher in groups C (88.9%) and D (86.7%) than in A (7.4%) and B (6.7%) (P < 0.05).
The Eag1 K(+) channel might be involved in the pathophysiological processes of PCa, and is expected to be a valuable target for the diagnosis and treatment of PCa.
研究Eag1钾离子通道在前列腺癌（PCa）组织中的表达情况，及其与PCa发生发展的相关性，探讨其是否可作为PCa诊断和治疗的靶点。
采用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法，检测雄激素依赖型PCa（ADPCa）（A组）和雄激素非依赖型PCa（AIPCa）（B组）正常瘤旁组织以及ADPCa（C组）和AIPCa（D组）肿瘤组织中Eag1钾离子通道的mRNA和蛋白表达。
A、B、C、D组Eag1钾离子通道mRNA表达相对系数分别为0.265±0.413、0.167±0.511、2.673±2.988和2.815±2.901，后两组显著升高（P<0.05）。C组（88.9%）和D组（86.7%）Eag1钾离子通道蛋白表达阳性率显著高于A组（7.4%）和B组（6.7%）（P<0.05）。
Eag1钾离子通道可能参与PCa的病理生理过程，有望成为PCa诊断和治疗的重要靶点。
