Lancto Cheryl A, Torres Sheila M F, Hendrickson Julie A, Martins Kyra V, Rutherford Mark S
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, 205 VSB, St Paul, MN 55108, USA.
Vet Dermatol. 2013 Aug;24(4):414-21, e90. doi: 10.1111/vde.12034. Epub 2013 May 24.
Reports indicate that human and canine patients with atopic dermatitis (AD) have reduced production of several skin antimicrobial peptides, but more recent data have called those results into question.
HYPOTHESIS/OBJECTIVES: To compare the mRNA expression of seven antimicrobial peptide genes in lesional and adjacent nonlesional skin biopsy specimens from dogs with AD with those from normal dogs and from dogs experiencing other inflammatory skin conditions.
Normal dogs and patients with AD or other inflammatory skin conditions were enrolled with owner permission and approval of the Institutional Animal Care and Use Committee.
Transcripts were measured by quantitative RT-PCR using a standard curve assessment.
Normal transcript levels for all seven antimicrobial peptides varied depending on the body site assessed. Transcripts for secretory leukocyte proteinase inhibitor (SLPI) and skin-derived antileucoproteinase (SKALP; also known as elafin) were typically ~10-fold greater in number than transcripts for the canine β-defensins (CBD)-1, -102, -103, -122 and -124. Transcripts for SKALP, SLPI, CBD-1, CBD-103 and CBD-122 were lower in both lesional and adjacent nonlesional skin from dogs with AD in comparison to normal skin. Transcripts were reduced to a similar extent versus normal dogs in skin of dogs with inflammatory skin conditions from both lesional and nonlesional biopsies, except for CBD-122, which was reduced only in lesional skin. Compared with normal dog skin, transcripts for CBD-102 and CBD-124 were unaffected in dogs with AD.
Both SKALP and SLPI may be important contributors to skin innate immunity, but their decreased expression in AD patients does not account for increased skin infections compared with other skin conditions.
报告显示，患有特应性皮炎（AD）的人类和犬类患者体内几种皮肤抗菌肽的生成减少，但最近的数据对这些结果提出了质疑。
假设/目的：比较患有AD的犬只与正常犬只以及患有其他炎症性皮肤病的犬只的病变皮肤活检标本和相邻非病变皮肤活检标本中7种抗菌肽基因的mRNA表达情况。
在获得主人许可并经机构动物护理和使用委员会批准后，纳入正常犬只以及患有AD或其他炎症性皮肤病的犬只。
使用标准曲线评估通过定量RT-PCR测量转录本。
所有7种抗菌肽的正常转录本水平因评估的身体部位而异。分泌型白细胞蛋白酶抑制剂（SLPI）和皮肤源性抗白细胞蛋白酶（SKALP；也称为弹性蛋白酶）的转录本数量通常比犬β-防御素（CBD）-1、-102、-103、-122和-124的转录本数量多约10倍。与正常皮肤相比，患有AD的犬只的病变皮肤和相邻非病变皮肤中SKALP、SLPI、CBD-1、CBD-103和CBD-122的转录本均较低。在患有炎症性皮肤病的犬只的病变和非病变活检皮肤中，与正常犬只相比，转录本减少程度相似，但CBD-122仅在病变皮肤中减少。与正常犬皮肤相比，患有AD的犬只中CBD-102和CBD-124的转录本未受影响。
SKALP和SLPI可能都是皮肤固有免疫的重要贡献者，但与其他皮肤病相比，它们在AD患者中的表达降低并不能解释皮肤感染增加的原因。
