Lohajaroensub Rajit, Sawangmake Chenphop, Rodkhum Channarong, Tuntivanich Nalinee
Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Veterinary Pharmacology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Vet Sci. 2022 Apr 21;9(5):200. doi: 10.3390/vetsci9050200.
The human amniotic membrane has been successfully used in human ocular reconstruction. Several studies have demonstrated its properties, including antimicrobial features. As a result of the restricted availability of human amniotic membrane for veterinary use, canine amniotic membrane has become an attractive alternative. Clinical studies of the application of canine amniotic membrane in animals and the understanding of its biological properties are limited. This study aimed to determine the expression of peptide genes of natural antimicrobials in canine amniotic membrane. Expressions of canine β-defensin 1, 102, and 103, and canine Elafin were determined in healthy puppies by real-time quantitative polymerase chain reaction. Canine β-defensin 1, 103, and Elafin were expressed in all samples, possibly suggesting a role in the innate immune system of normal canine amniotic membrane. Further investigations of protein expression and localization are recommended.
人羊膜已成功应用于人类眼部重建。多项研究证实了其特性，包括抗菌特性。由于用于兽医的人羊膜供应有限，犬羊膜已成为一种有吸引力的替代品。犬羊膜在动物中的应用临床研究及其生物学特性的了解有限。本研究旨在确定天然抗菌肽基因在犬羊膜中的表达。通过实时定量聚合酶链反应测定健康幼犬中犬β-防御素1、102和103以及犬弹性蛋白酶的表达。犬β-防御素1、103和弹性蛋白酶在所有样本中均有表达，这可能表明它们在正常犬羊膜的先天免疫系统中发挥作用。建议进一步研究蛋白质表达和定位。
