Materials Science and Engineering, CSIRO, Private Bag 10, Bayview Avenue, Clayton, Vic 3169, Australia.
Biomacromolecules. 2013 Jul 8;14(7):2305-16. doi: 10.1021/bm400430t. Epub 2013 Jun 10.
We show for the first time the possibility of using networks of amyloid fibrils, adsorbed to solid supports and with plasma polymer coatings, for the fabrication of chemically homogeneous surfaces with well-defined nanoscale surface features reminiscent of the topography of the extracellular matrix. The robust nature of the fibrils allows them to withstand the plasma polymer deposition conditions used with no obvious deleterious effect, thus enabling the underlying fibril topography to be replicated at the polymer surface. This effect was seen despite the polymer coating thickness being an order of magnitude greater than the fibril network. The in vitro culture of fibroblast cells on these surfaces resulted in increased attachment and spreading compared to flat plasma polymer films with the same chemical composition. The demonstrated technique allows for the rapid and reproducible fabrication of substrates with nanoscale fibrous topography that we believe will have applications in the development of new biomaterials allowing, for example, the investigation of the effect of extracellular matrix mimicking nanoscale morphology on cellular phenotype.
我们首次展示了使用吸附在固体支撑物上的淀粉样纤维网络并带有等离子体聚合物涂层的可能性，用于制造具有化学均一的纳米级表面特征的表面，这些表面特征类似于细胞外基质的形貌。纤维的坚固性质使它们能够承受使用的等离子体聚合物沉积条件，而没有明显的有害影响，从而能够在聚合物表面复制基础纤维的形貌。尽管聚合物涂层的厚度比纤维网络大一个数量级，但仍观察到了这种效果。与具有相同化学成分的平面等离子体聚合物膜相比，在这些表面上培养成纤维细胞导致细胞附着和铺展增加。所展示的技术允许快速且可重复地制造具有纳米纤维形貌的基底，我们相信这将在开发新的生物材料方面具有应用，例如，可以研究模仿细胞外基质纳米级形态对细胞表型的影响。
