Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Histopathology. 2013 Aug;63(2):279-86. doi: 10.1111/his.12153. Epub 2013 May 23.
To study prostate cancer zonal differences in TMPRSS2-ERG gene rearrangement.
We examined 136 well-characterized dominant anterior prostatic tumours, including 61 transition zone (TZ) and 75 anterior peripheral zone (PZ) lesions, defined using strict anatomical considerations. TMPRSS2-ERG FISH and ERG protein immunohistochemistry were performed on tissue microarrays. FISH results, available for 56 TZ and 71 anterior PZ samples, were correlated with ERG staining and TZ-associated 'clear cell' histology. Fewer TZ cancers (four of 56; 7%) were rearranged than anterior PZ cancers (18 of 71; 25%) (P = 0.009); deletion was the sole mechanism of TZ cancer rearrangement. ERG protein overexpression was present in 4% (two of 56; both FISH+) and 30% (21 of 71; 17 FISH+) of TZ and anterior PZ tumours, respectively. 'Clear cell' histology was present in 21 of 56 (38%) TZ and eight of 71 (11%) anterior PZ tumours. Seven per cent of cancers with and 21% without this histology had rearrangement, regardless of zonal origin.
TMPRSS2-ERG rearrangement occurs in dominant TZ and anterior PZ prostate cancers, with all rearranged TZ cancers in this cohort showing deletion. ERG immunohistochemistry demonstrated excellent sensitivity (86%) and specificity (96%) for TMPRSS2-ERG rearrangement. TMPRSS2-ERG fusion is rare in TZ tumours and present at a low frequency in tumours displaying 'clear cell' histology.
研究 TMPRSS2-ERG 基因重排在前列腺癌中的区带差异。
我们检查了 136 个特征明确的优势前叶前列腺肿瘤，包括 61 个移行区（TZ）和 75 个前周缘区（PZ）病变，这些病变是根据严格的解剖学考虑定义的。对组织微阵列进行了 TMPRSS2-ERG FISH 和 ERG 蛋白免疫组织化学检测。FISH 结果可用于 56 个 TZ 和 71 个前 PZ 样本，并与 ERG 染色和 TZ 相关的“透明细胞”组织学相关。TZ 癌的重排比例（56 例中有 4 例，7%）低于前 PZ 癌（71 例中有 18 例，25%）（P=0.009）；缺失是 TZ 癌重排的唯一机制。ERG 蛋白过表达分别存在于 4%（56 例中有 2 例，均为 FISH+）和 30%（71 例中有 21 例，17 例为 FISH+）的 TZ 和前 PZ 肿瘤中。21%（56 例中有 21 例）和 8%（71 例中有 8 例）的 TZ 和前 PZ 肿瘤分别存在“透明细胞”组织学。有和没有这种组织学的癌症中，7%有重排，而与区带起源无关。
TMPRSS2-ERG 重排在优势 TZ 和前 PZ 前列腺癌中发生，本队列中所有重排的 TZ 癌均显示缺失。ERG 免疫组织化学检测对 TMPRSS2-ERG 重排具有优异的敏感性（86%）和特异性（96%）。TZ 肿瘤中 TMPRSS2-ERG 融合罕见，表现为“透明细胞”组织学的肿瘤频率较低。
