Institute for Creative Leather Technologies, University of Northampton, Northampton, UK.
J Microsc. 2013 Jul;251(1):14-8. doi: 10.1111/jmi.12052. Epub 2013 May 23.
The properties and performance of collagen-based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three-dimensional (3-D) visualization on a PC and using a 3-D immersive technology system. The 3-D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.
基于胶原蛋白的材料的性能和特性可能会受到胶原蛋白纤维束的模式、方向和编织的影响。本研究的目的是开发并应用方法，使用苏木精和曙红染色的薄组织切片，通过共焦激光扫描显微镜来可视化真皮。对数据进行处理，以便在 PC 上和使用三维沉浸式技术系统进行三维可视化。共焦显微镜图像堆栈的三维可视化允许评估包括胶原蛋白纤维束在内的胶原大分子结构。所开发的方法为观察复杂的有机结构提供了一种新方法，在医学领域具有进一步的潜在应用。
