美国推荐的新生儿筛查疾病中干血斑标志物的稳定性。
The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States.
机构信息
Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30341, USA.
出版信息
Clin Biochem. 2011 Dec;44(17-18):1445-50. doi: 10.1016/j.clinbiochem.2011.09.010. Epub 2011 Sep 21.
Abstract
OBJECTIVE
We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples.
DESIGN AND METHODS
We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.
RESULTS
During the 30 ± 5 day studies, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high-humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and 4 of the 7 lost more than 50% of initial levels within the first week of storage.
CONCLUSIONS
Minimizing both humidity and temperature in DBS transportation and storage environments is essential to maintaining sample integrity.
摘要
目的
我们旨在分别测量热和湿度对干血斑（DBS）样本中 34 种先天缺陷标志物水平变化的影响。
设计和方法
我们将配对的 DBS 样本在低湿度和高湿度环境中于 37°C 下储存预定的时间间隔。在单个分析运行中测量每个完整样本集中所有样本的标志物水平。
结果
在 30±5 天的研究中，半乳糖-1-磷酸尿苷酰转移酶和生物素酶在低湿度储存中几乎失去了 65%的初始活性；27 种其他标志物的大部分降解归因于高湿度储存的不利影响；在高湿度储存的 DBS 中，7 种标志物在研究结束时损失了初始水平的 90%以上，其中 4 种标志物在储存的第一周内损失了初始水平的 50%以上。
结论
在 DBS 运输和储存环境中，尽量减少湿度和温度对于保持样本完整性至关重要。
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