Yang Jun, Peng Hua-Sheng, Xuan Yan, Qu Jie
Anhui Key Laboratory of Modernized Chinese Material Medica, Department of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
Zhong Yao Cai. 2012 Dec;35(12):1932-9.
To study the regular pattern of growing development and the structural changes of the main root of Dipsacus asper; Find the basis for distinguishing between wild and cultivated Dipsacus asper as well as identifying cultivated Dipsacus asper at different growth years.
Continuous sectional sampling and histological observation was conducted on the main roots of annual and biennial cultivated Dipsacus asper and on the main roots of bolting and non-bolting plants of wild Dipsacus asper; Histological observation was carried out respectively on the main roots of one to four-year-old cultivated Dipsacus asper.
It was discovered for the first time that there was structure of growth rings in the main roots of Dipsacus asper. And the ways in which the vessels of the growth rings were arranged differed between wild and cultivated Dipsacus asper, the vessels of growth rings in wild Dipsacus asper were arranged tangentially while those in cultivated Dipsacus asper were arranged as clusters. Pith existed in the upper part of roots of wild Dipsacus asper,while it was not found in the cultivated ones, and this could be adopted as a preliminary method to distinguish the wild Dipsacus asper from cultivatedone. The structures of growth rings in each main root were in consistency from top to bottom, and the thickening of roots mainly depended on the growth of xylem in the first year. There were particular relations and regular patterns between the structure of main roots of one to four-year-old cultivated Dipsacus asper and the ages of their growth, which could serve as the primary basis for identifying their growth years.
Methods to distinguish wild Dipsacus asper from cultivated ones at different ages by organization structure are established, which could provide the basis for identification of the medicinal material of Dipsacus asper.
研究续断主根的生长发育规律及结构变化；寻找区分野生与栽培续断以及鉴定不同生长年份栽培续断的依据。
对一年生和二年生栽培续断的主根以及野生续断抽薹和未抽薹植株的主根进行连续切片取样和组织学观察；对一至四年生栽培续断的主根分别进行组织学观察。
首次发现续断主根存在年轮结构。野生与栽培续断年轮中导管的排列方式不同，野生续断年轮导管呈切向排列，栽培续断年轮导管呈簇状排列。野生续断根的上部存在髓，栽培续断中未发现，可作为区分野生续断与栽培续断的初步方法。各主根年轮结构自上而下一致，根的加粗主要依赖于第一年木质部的生长。一至四年生栽培续断主根结构与其生长年龄之间存在特定关系和规律，可作为鉴定其生长年份的主要依据。
建立了通过组织结构区分不同年龄野生与栽培续断的方法，可为续断药材鉴定提供依据。
