Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, V,le Regina Elena 299, 00161 Rome, Italy.
J Transl Med. 2013 May 24;11:127. doi: 10.1186/1479-5876-11-127.
Virus-like particles (VLPs) from an Italian GII.4 norovirus strain were used to investigate activation and maturation of circulating antigen presenting cells (APCs) of human origin.
Peripheral blood mononuclear cells (PBMCs) isolated from five healthy subjects were pulsed ex vivo with VLPs, and stained with a set of monoclonal antibodies (MAbs) for phenotypic analysis by flow cytometry. Cytokine release in cell supernatants was investigated by ELISA.
Norovirus VLPs induced activation and maturation of circulating APCs derived from the five donors, as well as production of IL-6, IFN-γ and TNF-α cytokines.
The present results suggest that VLPs can activate antigen presenting cells for an efficient induction of the adaptive immune response.
来自意大利 GII.4 诺如病毒株的病毒样颗粒（VLPs）被用于研究人源性循环抗原呈递细胞（APC）的激活和成熟。
从五名健康受试者中分离外周血单核细胞（PBMCs），并通过与一组单克隆抗体（MAbs）孵育进行体外脉冲处理，通过流式细胞术进行表型分析。通过 ELISA 研究细胞上清液中细胞因子的释放。
诺如病毒 VLPs 诱导了来自五名供体的循环 APC 的激活和成熟，以及 IL-6、IFN-γ 和 TNF-α 细胞因子的产生。
本研究结果表明 VLPs 可激活抗原呈递细胞，从而有效诱导适应性免疫反应。
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