Lab, of Molecular Biology and Viral Oncogenesis & AIDS Reference Center, Istituto Nazionale Tumori "Fond, G, Pascale", Naples, Italy.
J Transl Med. 2010 Feb 19;8:18. doi: 10.1186/1479-5876-8-18.
Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines.
丙型肝炎病毒 (HCV) 是慢性肝炎的主要危险因素之一，可进展为肝硬化和肝细胞癌，也是 II 型混合性冷球蛋白血症 (MC) 的主要危险因素之一，后者可能进一步演变为显性 B 细胞非霍奇金淋巴瘤 (NHL)。先前已经表明，涉及 II 型 MC 以及与 HCV 相关的 NHL 的克隆 B 细胞表达的 B 细胞受体 (BCR) 库受到少数可变重 (VH)-和轻 (VL)-链基因的限制。在这些基因中，VK3-20 轻链独特型已被选为被动和主动免疫策略的可能靶标。在本研究中，我们描述了用 VK3-20 蛋白体外刺激人免疫细胞后先天和早期适应性免疫反应的多参数分析结果。通过实施高通量技术，如多参数流式细胞术和细胞因子和趋化因子的多重分析，实现了这一目标。
