三重 PCR 检测法用于快速鉴定 3 种主要弧菌，即霍乱弧菌、副溶血性弧菌和弗氏柠檬酸杆菌。
Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis.
机构信息
Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Gandhinagar, Gujarat 382 007, India.
出版信息
Diagn Microbiol Infect Dis. 2013 Aug;76(4):526-8. doi: 10.1016/j.diagmicrobio.2013.04.005. Epub 2013 May 22.
A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology.
建立了一种三重 PCR 检测方法，用于鉴定 3 种主要弧菌，即霍乱弧菌、副溶血性弧菌和弗氏弧菌，分别针对它们的溶血素、利用血红素和中央调控基因。该方法使用 227 个样本的细胞裂解物，具有简单、快速、敏感和特异的特点，在研究和流行病学中具有应用价值。
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