Department of Chemistry, Faculty of Science, Okayama University of Science, Ridaicho, Kita-Ku, Okayama 700-0005, Japan.
Int J Biol Macromol. 2013 Sep;60:39-44. doi: 10.1016/j.ijbiomac.2013.05.006. Epub 2013 May 21.
We demonstrated the immobilization of double-stranded DNA onto the glass beads by psoralen, one of the DNA-intercalators in nature. As a result, DNA-immobilized glass beads (DNA-P-beads) were prepared by the intercalation of psoralen, which was immobilized onto the glass surface, onto the double-stranded DNA. These DNA-P-beads formed covalent bondings between psoralen and the nucleic acid base by 365 nm UV irradiation. The amount of immobilized-DNA was 0.24 mg per gram of glass beads. These DNA-P-beads were stable in water, and the DNA on the bead surface maintained its double-stranded structure. These DNA-P-beads selectively removed the planar-structure containing harmful compounds, such as dioxin and polychlorinated biphenyl (PCB) derivatives, from an aqueous multi-component solution. Additionally, a DNA-P-bead column effectively removed harmful compounds. Furthermore, the DNA-P-bead column could be reused by the addition of common organic solvents, such as ethanol.
我们通过吖啶酮（一种天然的 DNA 嵌入剂）将双链 DNA 固定在玻璃珠上。结果，吖啶酮被固定在玻璃表面上，并插入双链 DNA 中，从而制备了 DNA 固定化玻璃珠（DNA-P 珠）。这些 DNA-P 珠通过 365nmUV 照射，在吖啶酮和核酸碱基之间形成共价键。每克玻璃珠固定的 DNA 量为 0.24 毫克。这些 DNA-P 珠在水中稳定，珠表面的 DNA 保持其双链结构。这些 DNA-P 珠从水多组分溶液中选择性地去除了含有有害物质的平面结构化合物，如二恶英和多氯联苯（PCB）衍生物。此外，DNA-P 珠柱可通过添加常见有机溶剂（如乙醇）而有效去除有害物质。此外，DNA-P 珠柱可以通过添加常见的有机溶剂（如乙醇）而重复使用。
