State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Laboratory of Food Microbial-Manufacturing Engineering, Jiangnan University, Wuxi 214122, China.
Metab Eng. 2013 Sep;19:10-6. doi: 10.1016/j.ymben.2013.05.002. Epub 2013 May 23.
The yeast Torulopsis glabrata CCTCC M202019, which is used for industrial pyruvate production, was chosen to explore the suitability of engineering this multi-vitamin auxotrophic yeast for increased malate production. Various metabolic engineering strategies were used to manipulate carbon flux from pyruvate to malate: (i) overexpression of pyruvate carboxylase and malate dehydrogenase; (ii) identification of the bottleneck in malate production by model iNX804; (iii) simultaneous overexpression of genes RoPYC, RoMDH and SpMAE1. Using these strategies, 8.5gL(-1) malate was accumulated in the engineered strain T.G-PMS, which was about 10-fold greater than that of the control strain T.G-26. The results presented here suggest that T. glabrata CCTCC M202019 is a promising candidate for industrial malate production.
选择用于工业丙酮酸生产的酵母 Torulopsis glabrata CCTCC M202019，以探索工程改造这种多维生素营养缺陷型酵母以提高苹果酸产量的适宜性。采用各种代谢工程策略来操纵从丙酮酸到苹果酸的碳通量：（i）过表达丙酮酸羧化酶和苹果酸脱氢酶；（ii）通过模型 iNX804 确定苹果酸生产的瓶颈；（iii）同时过表达基因 RoPYC、RoMDH 和 SpMAE1。使用这些策略，工程菌株 T.G-PMS 中积累了 8.5gL(-1)的苹果酸，比对照菌株 T.G-26 高约 10 倍。这里呈现的结果表明，CCTCC M202019 是工业苹果酸生产的有前途的候选菌株。
