具有 RGD/YIGSR 肽的槽型 PLGA 薄膜在骨骼肌组织工程中的潜在应用。
Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering.
机构信息
Department of Chemical Engineering, National Taiwan University, No.1, Roosevelt Rd., Sec. 4, Taipei 106, Taiwan.
出版信息
Colloids Surf B Biointerfaces. 2013 Oct 1;110:88-95. doi: 10.1016/j.colsurfb.2013.04.016. Epub 2013 Apr 26.
Alignment of myocytes or myotubes is critical for skeletal muscle tissue engineering. In this study, grooved PLGA films (800nm in width of ridge/groove and 600nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for skeletal muscle tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for skeletal muscle tissue engineering.
肌细胞或肌管的对齐对于骨骼肌组织工程至关重要。在这项研究中，制备了具有 RGD 或 YIGSR 肽的槽型 PLGA 薄膜（脊/槽宽度为 800nm，深度为 600nm），以评估其用于骨骼肌组织工程的效果。与未经处理的 PLGA 对照相比，RGD 或 YIGSR 的呈现促进了 C2C12 成肌细胞的生长和分化。另一方面，细胞形态受到槽型结构的引导，即肌母细胞和肌管与槽的方向对齐。本研究阐明了表面生物化学和形貌线索对可生物降解聚合物薄膜上 C2C12 成肌细胞增殖和分化的影响。表面形貌和肽呈现的组合在设计骨骼肌组织工程支架方面具有巨大潜力。
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