Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
Plant Mol Biol. 2013 Oct;83(3):191-204. doi: 10.1007/s11103-013-0079-3. Epub 2013 May 25.
It is becoming increasingly evident that the RNA degradome is a crucial component of the total cellular RNA pool. Here, we present an analysis of the medium-sized RNAs (midi RNAs) that form in Arabidopsis thaliana. Our analyses revealed that the midi RNA fraction contained mostly 20-70-nt-long fragments derived from various RNA species, including tRNA, rRNA, mRNA and snRNA. The majority of these fragments could be classified as stable RNA degradation intermediates (RNA degradants). Using two dimensional polyacrylamide gel electrophoresis, we demonstrated that high copy number RNA (hcn RNA) degradants appear in plant cells not only during stress, as it was earlier suggested. They are continuously produced also under physiological conditions. The data collected indicated that the accumulation pattern of the hcn RNA degradants is organ-specific and can be affected by various endogenous and exogenous factors. In addition, we demonstrated that selected degradants efficiently inhibit translation in vitro. Thus, the results of our studies suggest that hcn RNA degradants are likely to be involved in the regulation of gene expression in plants.
越来越明显的是，RNA 降解组是总细胞 RNA 库的重要组成部分。在这里，我们对拟南芥中形成的中型 RNA（midi RNA）进行了分析。我们的分析表明，midi RNA 部分主要包含来自各种 RNA 物种的 20-70-nt 长片段，包括 tRNA、rRNA、mRNA 和 snRNA。这些片段中的大多数可以归类为稳定的 RNA 降解中间体（RNA 降解物）。通过二维聚丙烯酰胺凝胶电泳，我们证明了高拷贝数 RNA（hcn RNA）降解物不仅在应激条件下，如之前所建议的那样，在植物细胞中出现。它们在生理条件下也在持续产生。收集的数据表明，hcn RNA 降解物的积累模式具有器官特异性，并可能受到各种内源性和外源性因素的影响。此外，我们还证明了选定的降解物能够有效地抑制体外翻译。因此，我们的研究结果表明，hcn RNA 降解物可能参与植物基因表达的调控。
