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应用胚胎侧胸皮片培养中微珠植入技术研究胚胎乳腺发育的分子机制- 实验方案

Investigating molecular mechanisms of embryonic mammary gland development by bead-implantation in embryonic flank explant cultures - a protocol.

机构信息

Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Singapore 138673, Singapore.

出版信息

J Mammary Gland Biol Neoplasia. 2013 Jun;18(2):247-52. doi: 10.1007/s10911-013-9297-1. Epub 2013 May 26.

DOI:10.1007/s10911-013-9297-1
PMID:23709170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3691484/
Abstract

The involvement of molecular mechanisms in a particular process such as embryonic mammary gland development, can be revealed by modulation of one or several factors that purportedly act in that process. If those factors or their inhibitors are soluble, their function can be tested by loading them onto small inert beads, which are then implanted in cultured explants of the tissue of interest, in this case embryonic flanks. We here describe a protocol for such experiments.

摘要

通过调节一个或几个据称在特定过程中起作用的因素，可以揭示分子机制在特定过程中的作用。如果这些因素或它们的抑制剂是可溶的，它们的功能可以通过将它们加载到小的惰性珠上进行测试，然后将这些珠植入感兴趣的组织（在这种情况下是胚胎侧翼）的培养外植体中。我们在这里描述了这样的实验的方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/658f/3691484/1b7e297c7670/10911_2013_9297_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/658f/3691484/1b7e297c7670/10911_2013_9297_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/658f/3691484/1b7e297c7670/10911_2013_9297_Fig1_HTML.jpg

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Ectodysplasin/NF-κB signaling in embryonic mammary gland development.外胚层发育不良/NF-κB 信号通路在胚胎期乳腺发育中的作用。
J Mammary Gland Biol Neoplasia. 2013 Jun;18(2):165-9. doi: 10.1007/s10911-013-9277-5. Epub 2013 Apr 17.
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整合素β4对甲状旁腺激素相关蛋白（PTHrP）的调节是其对乳腺发育起作用的基础。
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