Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 project, Oral Science Research Institute, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.
J Mammary Gland Biol Neoplasia. 2013 Jun;18(2):233-7. doi: 10.1007/s10911-013-9292-6. Epub 2013 May 11.
To reveal the specific functions of various genes during embryonic development, the manipulation of genes using techniques such as electroporation is of fundamental importance for providing direct evidence concerning function or downstream activation of signaling networks. In vitro embryo culture and electroporation are useful techniques to introduce foreign genes, for developmental biology studies. Among the various mammalian culture techniques, Trowell culture is suitable for studies of embryonic mammary gland development because of its stability and ease of use in conjunction with electroporation technique application. The manipulation of gene expression using electroporation is a useful technique for the functional analysis of a particular gene. In this protocol, full steps for electroporation and in vitro embryo culture have been described for use in embryonic mammary gland development research.
为了揭示胚胎发育过程中各种基因的具体功能，使用电穿孔等技术对基因进行操作对于提供有关信号转导网络功能或下游激活的直接证据至关重要。体外胚胎培养和电穿孔是引入外源基因的有用技术，可用于发育生物学研究。在各种哺乳动物培养技术中，由于其稳定性和与电穿孔技术应用相结合的易用性，Trowell 培养适合于胚胎乳腺发育的研究。使用电穿孔来操纵基因表达是分析特定基因功能的有用技术。在本方案中，已详细描述了电穿孔和体外胚胎培养的完整步骤，可用于胚胎乳腺发育研究。
