Sohn Woon-Mok, Chai Jong-Yil, Na Byoung-Kuk, Yong Tai-Soon, Eom Keeseon S, Park Hansol, Min Duk-Young, Rim Han-Jong
Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
Korean J Parasitol. 2013 Apr;51(2):191-6. doi: 10.3347/kjp.2013.51.2.191. Epub 2013 Apr 25.
The echinostome metacercariae encysted in Cipangopaludina sp. snails that were purchased from a market in Vientiane Municipality, Lao PDR, were identified as Echinostoma macrorchis (Digenea: Echinostomatidae) through recovery of adult flukes after experimental infection to rats and a cat. The metacercariae were round, 113-128 (121)×113-125 (120) µm, having a thick cyst wall, a head collar armed with collar spines, and excretory granules. The adult flukes recovered from the rats and cat at day 14 and 30 post-infection, respectively, were elongated, ventrally curved, and 3.9-6.3×0.7-1.1 mm in size. The head collar was distinct, bearing 43-45 collar spines with 5 angle spines on each side. Two testes were large (as the name implies), tandem, and slightly constricted at the middle, with irregular margins. Eggs were operculated, ovoid to elliptical, and 88-95×56-60 µm. In scanning electron microscopy, the head collar was prominent, with 43-45 collar spines. Scale-like tegumental spines were densely distributed on the ventral surface between the oral and ventral suckers. Sensory papillae were distributed mainly on the tegument around the 2 suckers. It is confirmed that E. macrorchis is distributed in Lao PDR using Cipangopaludina sp. snails as the second intermediate host.
从老挝万象市一个市场购买的包埋于圆田螺中的棘口吸虫囊蚴，经实验感染大鼠和猫后获得成虫，鉴定为巨睾棘口吸虫（复殖目：棘口科）。囊蚴呈圆形，大小为113 - 128（121）×113 - 125（120）µm，具有厚的囊壁、带有颈棘的头冠和排泄颗粒。分别在感染后第14天和30天从大鼠和猫体内回收的成虫呈细长形，腹面弯曲，大小为3.9 - 6.3×0.7 - 1.1 mm。头冠明显，有43 - 45个颈棘，每侧有5个角棘。两个睾丸大（顾名思义），呈串联状，中部略有缢缩，边缘不规则。虫卵有卵盖，呈卵形至椭圆形，大小为88 - 95×56 - 60 µm。在扫描电子显微镜下，头冠突出，有43 - 45个颈棘。鳞状体表棘密集分布于口吸盘和腹吸盘之间的腹面。感觉乳突主要分布在两个吸盘周围的体表。证实巨睾棘口吸虫在老挝以圆田螺作为第二中间宿主分布。
