Division of Environmental Engineering for Symbiosis, Graduate School of Engineering, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577, Japan.
Archaea. 2013;2013:723871. doi: 10.1155/2013/723871. Epub 2013 Apr 22.
Archaeal 16S rRNA gene compositions and environmental factors of four distinct solfataric acidic hot springs in Kirishima, Japan were compared. The four ponds were selected by differences of temperature and total dissolved elemental concentration as follows: (1) Pond-A: 93°C and 1679 mg L(-1), (2) Pond-B: 66°C and 2248 mg L(-1), (3) Pond-C: 88°C and 198 mg L(-1), and (4) Pond-D: 67°C and 340 mg L(-1). In total, 431 clones of 16S rRNA gene were classified into 26 phylotypes. In Pond-B, the archaeal diversity was the highest among the four, and the members of the order Sulfolobales were dominant. The Pond-D also showed relatively high diversity, and the most frequent group was uncultured thermoacidic spring clone group. In contrast to Pond-B and Pond-D, much less diverse archaeal clones were detected in Pond-A and Pond-C showing higher temperatures. However, dominant groups in these ponds were also different from each other. The members of the order Sulfolobales shared 89% of total clones in Pond-A, and the uncultured crenarchaeal groups shared 99% of total Pond-C clones. Therefore, species compositions and biodiversity were clearly different among the ponds showing different temperatures and dissolved elemental concentrations.
比较了日本雾岛的四个不同的沸腾酸性温泉的古菌 16S rRNA 基因组成和环境因素。这四个池塘是根据温度和总溶解元素浓度的差异选择的：（1）池塘 A：93°C 和 1679mg/L，（2）池塘 B：66°C 和 2248mg/L，（3）池塘 C：88°C 和 198mg/L，和（4）池塘 D：67°C 和 340mg/L。总共对 431 个 16S rRNA 基因克隆进行了分类，分为 26 个类群。在池塘 B 中，古菌多样性在四个池塘中最高，硫磺酸杆菌目成员占优势。池塘 D 也表现出相对较高的多样性，最常见的组是未培养的嗜热酸性泉克隆群。与池塘 B 和池塘 D 相比，温度较高的池塘 A 和池塘 C 中检测到的古菌克隆多样性要少得多。然而，这些池塘中的优势群体也彼此不同。池塘 A 中的硫磺酸杆菌目成员占总克隆数的 89%，池塘 C 中的未培养的泉古菌群占总克隆数的 99%。因此，在表现出不同温度和溶解元素浓度的池塘中，物种组成和生物多样性明显不同。
