Faculty of Environmental Engineering, The University of Kitakyushu.
Department of Earth and Planetary Sciences, Faculty of Science, Kyushu University.
Microbes Environ. 2021;36(4). doi: 10.1264/jsme2.ME21048.
A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×10 microbes mL. The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were Proteobacteria and Aquificae (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan.
在日本秋田县大泷峡周围的高温温泉中发现了一个新的深分枝古菌谱系。活跃沸腾的温泉水中含有>1×10 个微生物 mL。通过分析 16S rRNA 基因扩增子评估微生物群落组成，结果表明，在 2016 年和 2019 年采集的样本中，优势细菌门分别为变形菌门和泉古菌门（>50%的微生物组成）。在这两年获得的读数中，约有 10%未被分配到任何分类单元。使用克隆文库和系统发育树确定的未分配序列的更详细的系统发育位置表明，它们形成了一个分支，与已知的门独立，亲缘关系较远，与现有数据库中的所有其他序列的相似度<82%。本研究结果表明，这种新型古菌门水平的谱系在日本的沸腾温泉中大量存在。
