Laboratory of Phytopathology, Wageningen University, 6708 PB, Wageningen, the Netherlands.
Mol Plant Pathol. 2013 Sep;14(7):719-27. doi: 10.1111/mpp.12042. Epub 2013 May 27.
The recognition of pathogen effectors by plant immune receptors leads to the activation of immune responses that often include a hypersensitive response (HR): rapid and localized host cell death surrounding the site of attempted pathogen ingress. We have demonstrated previously that the recognition of the Verticillium dahliae effector protein Ave1 by the tomato immune receptor Ve1 triggers an HR in tomato and tobacco. Furthermore, we have demonstrated that tomato Ve1 provides Verticillium resistance in Arabidopsis upon Ave1 recognition. In this study, we investigated whether the co-expression of Ve1 and Ave1 in Arabidopsis results in an HR, which could facilitate a forward genetics screen. Surprisingly, we found that the co-expression of Ve1 and Ave1 does not induce an HR in Arabidopsis. These results suggest that an HR may occur as a consequence of Ve1/Ave1-induced immune signalling in tomato and tobacco, but is not absolutely required for Verticillium resistance.
植物免疫受体识别病原体效应子会引发免疫反应，通常包括过敏反应（HR）：在病原体入侵的部位周围，宿主细胞迅速且局部死亡。我们之前已经证明，番茄免疫受体 Ve1 识别黄萎病菌效应蛋白 Ave1 会触发番茄和烟草中的 HR。此外，我们还证明了在识别 Ave1 后，番茄 Ve1 赋予拟南芥对黄萎病菌的抗性。在这项研究中，我们研究了在拟南芥中共同表达 Ve1 和 Ave1 是否会导致 HR，这可能有助于正向遗传学筛选。令人惊讶的是，我们发现 Ve1 和 Ave1 的共表达不会在拟南芥中诱导 HR。这些结果表明，HR 可能是 Ve1/Ave1 诱导的番茄和烟草免疫信号的结果，但不是黄萎病抗性所必需的。
