Verticillium wilt is a soil-borne disease that can cause devastating losses in cotton production. Because there is no effective chemical means to combat the disease, the only effective way to control Verticillium wilt is through genetic improvement. Therefore, the identification of additional disease-resistance genes will benefit efforts toward the genetic improvement of cotton resistance to Verticillium wilt. Based on screening of a BAC library with a partial homologous fragment and expression analysis, a -induced gene, , was isolated and cloned from the Verticillium wilt-resistant cotton cultivar Hai7124. The gene was located in the gene cluster containing and and adjacent to the Verticillium wilt-resistance QTL hotspot. was induced by Kleb and by the plant hormones salicylic acid (SA), methyl jasmonate (MeJA) and ethephon (ETH) but not by abscisic acid (ABA). Gbvdr6 was localized to the plasma membrane. Overexpression of in and cotton enhanced resistance to . Moreover, the JA/ET signaling pathway-related genes and the SA-related genes and were constitutively expressed in transgenic plants. -overexpressing was less sensitive than the wild-type plant to MeJA. Furthermore, the accumulation of reactive oxygen species and callose was triggered at early time points after infection. These results suggest that confers resistance to through regulation of the JA/ET and SA signaling pathways.