Post Graduate and Research Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore - 48, Tamil Nadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Sep;113:10-4. doi: 10.1016/j.saa.2013.04.083. Epub 2013 May 3.
The biosynthesis of silver nanoparticles and its antimicrobial property was studied using bacteria isolated from Spirulina products. Isolated bacteria were identified as Bacillus sp. MSK 1 (JX495945), Staphylococcus sp. MSK 2 (JX495946), Bacillus sp. MSK 3 (JX495947) and Brevundimonas sp. MSK 4 (JX495948). Silver nanoparticles (AgNPs) were synthesized using bacterial culture filtrate with AgNO3. The initial syntheses of Ag nanoparticles were characterized by UV-vis spectrophotometer (by measuring the color change to intense brown). Fourier Transform Infrared Spectroscopy (FTIR) study showed evidence that proteins are possible reducing agents and Energy-dispersive X-ray (EDX) study showing the metal silver as major signal. The structure of AgNPs was determined by Scanning electron microscopy (SEM) and X-ray diffraction (XRD). Synthesized Ag nanoparticles with an average size of 40-65 nm have antimicrobial property against human pathogens like Proteus vulgaris, Salmonella typhi, Vibrio cholera, Streptococcus sp., Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. Among the isolates Brevundimonas sp. MSK 4 alone showed good activity in both synthesis of AgNPs and antimicrobial activity. This work demonstrates the possible use of biological synthesized silver nanoparticles to combat the drug resistant problem.
采用从螺旋藻产品中分离出的细菌研究了银纳米粒子的生物合成及其抗菌性能。分离出的细菌被鉴定为芽孢杆菌 MSK 1（JX495945）、葡萄球菌 MSK 2（JX495946）、芽孢杆菌 MSK 3（JX495947）和短波单胞菌 MSK 4（JX495948）。使用细菌培养液和 AgNO3 合成了银纳米粒子（AgNPs）。Ag 纳米粒子的初始合成通过紫外可见分光光度计（通过测量颜色变为强烈的棕色）进行表征。傅里叶变换红外光谱（FTIR）研究表明，蛋白质可能是还原剂，能量色散 X 射线（EDX）研究表明金属银是主要信号。AgNPs 的结构通过扫描电子显微镜（SEM）和 X 射线衍射（XRD）确定。合成的 AgNPs 平均尺寸为 40-65nm，对人体病原体如普通变形杆菌、伤寒沙门氏菌、霍乱弧菌、链球菌、枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌具有抗菌活性。在分离株中，只有短波单胞菌 MSK 4 同时在 AgNPs 的合成和抗菌活性方面表现出良好的活性。这项工作证明了生物合成的银纳米粒子在对抗耐药性问题方面的可能用途。
